with a fresh ounce or so; this, as a rule, sufficing to completely
exhaust it of its resin. Wynter Blyth says that the red resin, or bixin,
is soluble in 25 parts of hot alcohol. It appears from these experiments
that much more is required to dissolve it out of commercial annatto.

The full process followed consisted in determining the moisture by
drying 100 grains at 212° F. till constant, and taking this dried
portion for estimation of the resin in the way just stated. The
alcoholic extract was evaporated to dryness over a water-bath, the
residue dissolved in solution of sodium carbonate, and the resin
precipitated by dilute sulphuric acid (these reagents being chosen as
the best after numerous trials with others), added in the slightest
possible excess. The resin was collected on a tared double filter paper,
washed with distilled water until the washings were entirely colorless,
dried and weighed.

The ash was found in the usual way, and the extractive by the
difference. In the ash the amount soluble was determined, and
qualitatively examined, as was the insoluble portion in most of them.

The results are as follows:

| 1. | 2. | 3. | 4. | 5. | 6. | 7. | 8. | 9. | 10.
Moisture | 21.75| 21.60| 20.39| 69.73| 18.00| 18.28| 15.71| 38.18| 19.33| 22.50
Resin | 3.00| 2.90| 1.00| 8.80| 3.00| 1.80| 5.40| 12.00| 5.90| 9.20
Extrac-
tive | 57.29| 59.33| 65.00| 19.47| 58.40| 65.67| 26.89| 20.82| 23.77| 28.50
Ash | 17.96| 16.17| 13.61| 2.00| 20.60| 14.25| 52.00| 29.00| 51.00| 39.80
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|100.00|100.00|100.00|100.00|100.00|100.00|100.00|100.00|100.00|100.00