All these reactions show that the phenol occurs in the free state in
the cones of this plant. In the same manner I treated the acicular
leaves, and portions of the stem separately, both being previously cut
up into small pieces, and from both I obtained phenol.

I have ascertained the relative amount of phenol in each part of the
plant operated upon; by heating the stem with water at 80° C., and
filtering, and repeating this operation until the aqueous filtrate
gave no violet color with ferric chloride and no white precipitate
with bromine water.

I found various quantities according to the age of the stem. The older
portions yielding as much as 0.1021 per cent, while the young portions
only gave 0.0654 per cent. The leaves yielding according to their age,
0.0936 and 0.0315 per cent.; and the cones also gave varying amounts,
according to their maturity, the amounts varying between 0.0774 and
0.0293.

Two methods were used in the quantitative estimation of the amount of
phenol. The first was the new volumetric method of M. Chandelon
(_Bulletin de la Societe Chemique de Paris_, July 20, 1882; and
_Deutsch-Americanishe Apotheker Zeitung_, vol. iii., No. 12, September
1, 1882), which I have found to be very satisfactory. The process
depends on the precipitation of phenol by a dilute aqueous solution of
bromine as tribromophenol. The second method was to extract, as
already staled, a known weight of each part of the plant with water,
until the last extract gives _no_ violet color with ferric chloride,
and no white precipitate with the bromine test (which is capable of
detecting in a solution the 1/60000 part of phenol). The aqueous
extract is at this point evaporated, then ether is added, and finally